Molecular identification of Wolbachia from the filarial nematode Mansonella perstans
Identifieur interne : 006A17 ( Main/Exploration ); précédent : 006A16; suivant : 006A18Molecular identification of Wolbachia from the filarial nematode Mansonella perstans
Auteurs : Paul B. Keiser [États-Unis] ; Yaya Coulibaly ; Joseph Kubofcik [États-Unis] ; Abdallah A. Diallo ; Amy D. Klion [États-Unis] ; Sekou F. Traoré ; Thomas B. Nutman [États-Unis]Source :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 2008.
Abstract
Wolbachiae are bacterial endosymbionts of insects and many filarial nematodes whose products trigger inflammation in filarial infections. The dependence of the parasites on their endosymbionts has also led to the use of antibiotics directed against the Wolbachiae, therapy that has been demonstrated to have a profound salutary effect on filarial infections. The identification of Wolbachiae in Mansonella species has been conclusively shown for
Url:
DOI: 10.1016/j.molbiopara.2008.04.012
PubMed: 18538871
PubMed Central: 2497444
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p id="P1">Wolbachiae are bacterial endosymbionts of insects and many filarial nematodes whose products trigger inflammation in filarial infections. The dependence of the parasites on their endosymbionts has also led to the use of antibiotics directed against the Wolbachiae, therapy that has been demonstrated to have a profound salutary effect on filarial infections. The identification of Wolbachiae in Mansonella species has been conclusively shown for <italic>Mansonella ozzardi</italic>
(Mo), but not for <italic>Mansonella perstans (Mp)</italic>
Using primers known to amplify the 16S ribosomal DNA of other filarial Wolbachiae, an identical 1393 bp band was found in all samples tested. Sequence analysis of these samples demonstrated a single consensus sequence for Mp Wolbachia 16S rDNA that was most similar to Wolbachia sequences from other filarial nematodes. When aligned with the only other Mansonella Wolbachia sequence (Mo) there were only 8 nucleotide differences in the 1369 bp overlapping sequence. Phylogenetic dendrograms, showing the relationship of the Mp Wolbachia to other Wolbachia 16S rDNA, tracked almost identically to the 5S rRNA of their parasite host. Wolbachia surface protein (WSP) was also demonstrated in protein extracted from Mp-containing whole blood. In advance of a treatment trial of Mp, a method for the quantitation of Mp Wolbachia was developed and used to demonstrate not only a relationship between microfilarial numbers and Wolbachia copy numbers, but also to demonstrate the effect of antibiotic on ridding Mp of Wolbachia.</p>
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